DYKDDDDK Peptide：A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site

产品概述

Synonyms ：DYKDDDDK tag;Flag-tag

同义词 ：DYKDDDDK 标签；Flag-tag

Description：

A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide.

描述：

 一种融合标签，称为DYKDDDK，由八个氨基酸（Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys）组成，包括一个肠激酶切割位点，专为免疫亲和色谱设计。它允许在非变性条件下洗脱。针对该肽的几个抗体已被开发出来。一种称为M1的抗体在双价金属阳离子存在下结合该肽，优选Ca2+。通过螯合剂实现洗脱。另一种策略是使用过量的游离DYKDDDK肽进行竞争性洗脱。

Sequence：  

Sequence： Shortening DYKDDDDK

Molecular： Formula C₄₁H₆₀N₁₀O₂₀ 

Molecular Weight ：1012.97

产品属性

Purity ：> 95% 

Solubility ：Soluble in Ultrapure water under 1mg/ml 

Form： Lyophilized 

Storage ：Store at -20°C. 

纯度： > 95% 

溶解性 ：可溶于超纯水，浓度小于1毫克/毫升

形态 ：冻干粉

储存 ：温度保持在-20°C。