漠宝生物科技
Make Research Easy
服务热线：0592-5771844
Make Research Easy
服务热线：0592-5771844
产品型号：A00186
更新时间：2026-07-17
厂商性质：代理商
访 问 量 ：3344
0592-5771844
THE™ 代表了"THE unmistakable, THE irreplaceable, THE best quality"。金斯瑞选择稳定、高产的杂交瘤细胞克隆，然后使用相应的抗原进行筛选分离，制备出特异性高、亲和力强的THE™ 系列金牌抗体。
您在实验中是否曾经因为样品量小、标签只有部分暴露、融合蛋白变性或其它棘手的问题而感到烦恼？金斯瑞THE™ 系列金牌抗体，让您的这些烦恼迎刃而解！
同时，我们也为您提供其它优质的免疫学相关产品。如凭借**的多肽合成能力，我们能够提供大多数抗体的封闭肽，让您确认观察到的信号是由抗体与靶抗原之间的相互作用产生，而不是由于非特异性结合而产生。
灵敏度—比市场上其他畅销的抗体更灵敏；
特异性—无交叉反应；
亲和力—-低解离常数Kd；
识别能力—能够识别并结合广泛的物种及表达系统的靶抗原；
低背景—无其它反应背景；
便捷—适用范围广，产品全面；
稳定—-好的批次间一致性，冻干状态下，可至少稳定保存2年。
Description | Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag. THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody. THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins. THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells. |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells |
| Purification | Protein A affinity column |
| Subclass | IgG1, k |
| Clone ID | 6G2A9 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Specificity | THE™ His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Note | GenScript can offer this product according to your requirement, including product size, buffer components,etc. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western Blot: 0.1-0.2 µg/ml Immunoprecipitation (IP): 1 µg/ml Immunofluorescent staining: 1 µg/ml Flow cytometry (FACS): 1 µg/ml TR-FRET assays: user-optimized Other applications: user-optimized |
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