His抗体A00186 Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags.

一、His抗体A00186产品介绍：

Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag.

THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody.

THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins.

THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.

中文：

特异于6个His标签的单克隆抗体可以极大地提高几种不同免疫分析的有效性，帮助研究人员识别、检测和纯化细菌、昆虫细胞和哺乳动物细胞中的多His融合蛋白。然而，由于6XHis标签的免疫原性较差，因此需要与KLH或其他载体结合作为免疫原。经过数百个选择周期后，GenScript的研究人员成功地分离出一种针对他的标签的抗体。

这个™ His标记抗体mAb与小鼠（IgG1亚型）具有很高的亲和力。在GenScript上进行的测试显示抗体也能识别4xHis和5xHis标签。这意味着即使6xHis标签仅部分暴露，它仍将被该抗体识别和结合。

THTM His标记单克隆抗体由小鼠腹水制备，并通过蛋白A亲和柱纯化。该抗体识别天然和变性形式的合成多His和多His标记的融合蛋白。该产物与细菌、昆虫细胞和哺乳动物细胞中表达的融合蛋白反应。THTM-His标签单克隆抗体识别位于融合蛋白N端、C端和内部区域的His标签。

THTM-His标记单抗可用于培养细胞的Western印迹分析、斑点印迹分析、ELISA、免疫荧光染色和流式细胞术。

二、His抗体A00186产品特性：