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Anti-His Affinity Resin
产品简介:

GenScript Anti-His Affinity Resin is designed for purification of His-tagged protein.

产品型号:L00439

更新时间:2026-07-24

厂商性质:代理商

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产品介绍
产品概述
Product descriptionGenScript Anti‐His Affinity Resin is designed for purification of His‐tagged protein that is difficult for single Ni‐NTA Agarose purification. This product is made from THE™ anti‐His monoclonal antibody conjugated to agarose resin. It specifically binds to His‐tagged proteins in the samples. Proteins nonspecifically binding to the resin can be eliminated by washing. Finally, His‐tagged protein can be eluted by different methods. In addition to that, the resin can be used for immunoprecipitation of His‐tagged proteins.


产品属性
Product Content50% resin in TBS with 0.02% sodium azide
Beads Structure4% agarose
Beads Size45-165 μm, averagely 90 μm
Coupled AntibodyTHE™ His Tag Antibody, mAb, Mouse (Cat.No A00186)
Binding capacity≥0.6 mg His-tagged protein/ml Resin
SpecificityN‐terminal/C‐terminal/internal /4 x His/5 x His/6 x His‐tagged proteins
Elution MethodAcid, alkaline and peptide competitive elutions
Repeat UsageGenerally the resin can be recycled at least 4 times. If maintained properly, the resin may reach 10 recycle times with minor binding capability loss
Reagents CompatibilityCompatible with mostly used reagents at certain concentrations
ConsistencyLot‐to‐lot consistency proved by strict production procedure
StorageThis product is stable for 1 year if stored at 2°C~8°C from the date of manufacture. Do not store at 0°C or below 0°C to avoid freezing the resin.


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